4U6T
Crystal structure of the Clostridium histolyticum colH collagenase polycystic kidney disease-like domain 2a at 1.76 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2013-05-14 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 61 |
| Unit cell lengths | 88.330, 88.330, 123.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.040 - 1.760 |
| R-factor | 0.2099 |
| Rwork | 0.207 |
| R-free | 0.27150 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.229 |
| Data reduction software | d*TREK (9.9.9.4 W9RSSI) |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.040 | 65.040 | 1.820 |
| High resolution limit [Å] | 1.760 | 3.790 | 1.760 |
| Rmerge | 0.068 | 0.059 | 0.374 |
| Total number of observations | 254417 | 29281 | 10867 |
| Number of reflections | 53717 | ||
| <I/σ(I)> | 11.5 | 23.3 | 2.3 |
| Completeness [%] | 99.5 | 99.6 | 95.7 |
| Redundancy | 4.7 | 5.18 | 2.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 290 | 3 M ammonium sulfate, 0.1 M MES pH 4.5, 15% (w/v) PEG 4000 |
