4U6M
Crystal structure of DNA/RNA duplex obtained in the presence of Spermine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 49.090, 49.090, 45.990 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.520 - 1.900 |
Rwork | 0.206 |
R-free | 0.23340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4u6l |
RMSD bond length | 0.006 |
RMSD bond angle | 1.163 |
Data reduction software | CrystalClear |
Data scaling software | d*TREK |
Phasing software | PHENIX |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.520 | 42.520 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.040 | 0.020 | 0.358 |
Total number of observations | 51463 | 5495 | 5139 |
Number of reflections | 5022 | ||
<I/σ(I)> | 23.2 | 80.9 | 5.7 |
Completeness [%] | 99.8 | 98.8 | 99.8 |
Redundancy | 10.17 | 10.67 | 10.25 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Na Cacodylate, Spermine tetrahydrochloride |