4U6F
Crystal structure of T-2 toxin bound to the yeast 80S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 436.640, 287.690, 304.390 |
| Unit cell angles | 90.00, 98.98, 90.00 |
Refinement procedure
| Resolution | 300.660 - 3.100 |
| R-factor | 0.1988 |
| Rwork | 0.198 |
| R-free | 0.24670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3U5B 3U5C 3U5D 3U5E 3U5F 3U5G 3U5H 3U5I |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.513 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX ((phenix.refine: dev_1702)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 300.660 | 3.000 | |
| High resolution limit [Å] | 2.900 | 400.000 | 2.900 |
| Rmerge | 0.401 | 3.839 | |
| Rmeas | 0.436 | 4.259 | |
| Total number of observations | 9178598 | ||
| Number of reflections | 1614049 | 155367 | |
| <I/σ(I)> | 4.19 | 0.49 | |
| Completeness [%] | 98.8 | 98.5 | |
| Redundancy | 5.69 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine |
