4U6E

HsMetAP in complex with (amino(phenyl)methyl)phosphonic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-03-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	47.433, 77.209, 48.379
Unit cell angles	90.00, 91.12, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.215
Rwork	0.212
R-free	0.26400
RMSD bond length	0.007
RMSD bond angle	1.065
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.850
Number of reflections	29959
<I/σ(I)>	2.09
Completeness [%]	99.6
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	15% PEG-MME 2000, 100 mM HEPES, pH=6.0, VAPOR DIFFUSION, temperature 298K