4U69
HsMetAP complex with (1-amino-2-methylpentyl)phosphonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-03-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.466, 77.264, 48.243 |
| Unit cell angles | 90.00, 91.23, 90.00 |
Refinement procedure
| Resolution | 16.820 - 1.600 |
| R-factor | 0.171 |
| Rwork | 0.170 |
| R-free | 0.20200 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.316 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 18.500 |
| High resolution limit [Å] | 1.570 |
| Number of reflections | 48478 |
| <I/σ(I)> | 3.4 |
| Completeness [%] | 99.6 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG 2000, potassium chloride, hepes, sodium chloride, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
