4U66

Induced Dimer Structure of Methionine Sulfoxide Reductase U16C from Clostridium Oremlandii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	102.564, 102.564, 227.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.820 - 2.900
R-factor	0.251
Rwork	0.248
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB DNTRY 4LWJ
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.950
High resolution limit [Å]	2.900	2.900
Number of reflections	26615
<I/σ(I)>	11.6
Completeness [%]	94.6	88.6
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	293	0.1M TRIS-HCL, 1.2M AMMONIUM SULFATE, 12%(V/V) GLYCEROL