4U5A

Sporozoite Protein for Cell Traversal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Collection date	2009-08-07
Detector	MARRESEARCH
Wavelength(s)	1.0072
Spacegroup name	P 21 21 21
Unit cell lengths	55.179, 64.316, 233.748
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.750
R-factor	0.2453
Rwork	0.242
R-free	0.27090
Structure solution method	MAD
RMSD bond length	0.004
RMSD bond angle	0.827
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.750	7.460	2.750
Rmerge	0.134	0.058	0.618
Total number of observations	251858
Number of reflections	22303
<I/σ(I)>	7.3
Completeness [%]	98.8	99.9	87.1
Redundancy	11.3	12.9	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	HEPES 7.5, magnesium chloride, PEG 3350
1	VAPOR DIFFUSION, SITTING DROP		298	HEPES 7.5, magnesium chloride, PEG 3350
1	VAPOR DIFFUSION, SITTING DROP		298	HEPES 7.5, magnesium chloride, PEG 3350