4U51
Crystal structure of Narciclasine bound to the yeast 80S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 435.340, 287.000, 303.360 |
Unit cell angles | 90.00, 98.98, 90.00 |
Refinement procedure
Resolution | 49.180 - 3.200 |
R-factor | 0.2053 |
Rwork | 0.204 |
R-free | 0.25250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3U5B 3U5C 3U5D 3U5E 3U5F 3U5G 3U5H 3U5I |
RMSD bond length | 0.012 |
RMSD bond angle | 1.590 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX ((phenix.refine: dev_1702)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.180 | 3.300 | |
High resolution limit [Å] | 3.200 | 40.000 | 3.200 |
Rmerge | 0.303 | 0.045 | 2.189 |
Rmeas | 0.331 | 0.057 | 2.385 |
Total number of observations | 7685659 | ||
Number of reflections | 1205730 | 256 | 106046 |
<I/σ(I)> | 6.47 | 12.41 | 0.91 |
Completeness [%] | 99.9 | 37.8 | 99.9 |
Redundancy | 6.46 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine |