4U4V

Structure of a nitrate/nitrite antiporter NarK in apo inward-open state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-11
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	35.504, 106.532, 124.609
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.151 - 2.350
R-factor	0.2051
Rwork	0.204
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.736
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.350
Number of reflections	18217
<I/σ(I)>	7.33
Completeness [%]	88.3
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.5	293	27% PEG 500DME, 100 mM ammonium nitrate, 100 mM MES-NaOH