4U4Q

Crystal structure of Homoharringtonine bound to the yeast 80S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	436.250, 286.920, 303.840
Unit cell angles	90.00, 98.90, 90.00

Refinement procedure

Resolution	49.690 - 3.000
R-factor	0.2088
Rwork	0.208
R-free	0.25460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3U5B 3U5C 3U5D 3U5E 3U5F 3U5G 3U5H 3U5I
RMSD bond length	0.012
RMSD bond angle	1.616
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: dev_1702))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.690		3.100
High resolution limit [Å]	3.000	40.000	3.000
Rmerge	0.341	0.062	3.527
Rmeas	0.361	0.072	3.725
Total number of observations	13621629
Number of reflections	1451516	280	134798
<I/σ(I)>	7.13	11.84	0.88
Completeness [%]	98.8	41.2	98.2
Redundancy	9.37

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine