4U4O

Crystal structure of Geneticin bound to the yeast 80S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	437.000, 286.750, 305.180
Unit cell angles	90.00, 99.24, 90.00

Refinement procedure

Resolution	135.579 - 3.600
R-factor	0.1913
Rwork	0.190
R-free	0.26670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3U5B 3U5C 3U5D 3U5E 3U5F 3U5G 3U5H 3U5I
RMSD bond length	0.018
RMSD bond angle	2.256
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: dev_1702))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	135.580		3.700
High resolution limit [Å]	3.600	150.000	3.600
Rmerge	0.517		3.303
Rmeas	0.535		3.432
Total number of observations	12887521
Number of reflections	855160		67248
<I/σ(I)>	5.77		0.96
Completeness [%]	100.0		100
Redundancy	13.52

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine