4U4H

Crystal Structure of HSV-1 UL21 N-terminal Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 63 2 2
Unit cell lengths	108.346, 108.346, 65.236
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.809 - 2.050
R-factor	0.1683
Rwork	0.166
R-free	0.22140
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.075
Data reduction software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.700	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.061
Number of reflections	14650
<I/σ(I)>	23.3	2.5
Completeness [%]	99.8	99.6
Redundancy	5.3	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	Ammonium Sulfate, HEPES, 2-methyl-2,4-pentanediol