4U4A
Complex Structure of BRCA1 BRCT with singly phospho Abraxas
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.900 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 187.500, 187.500, 85.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.890 - 3.510 |
R-factor | 0.3042 |
Rwork | 0.301 |
R-free | 0.36180 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.469 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 59.890 |
High resolution limit [Å] | 3.510 |
Number of reflections | 15491 |
<I/σ(I)> | 9.5 |
Completeness [%] | 100.0 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.01M Cobalt Chloride, 0.1 M(MES), pH 6.5, 30% PEG Mono Methyl Ether (MME) 5000 |