4U46
Crystal structure of an avidin mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-26 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.480, 79.800, 43.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.340 - 1.950 |
| R-factor | 0.18364 |
| Rwork | 0.182 |
| R-free | 0.21402 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wbi |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.638 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.070 | 0.520 |
| Number of reflections | 19264 | |
| <I/σ(I)> | 20 | 5 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | Well solution: 0.2 M sodium chloride, 0.1 M sodium cacodylate, 2.0 M ammonium sulphate |
