4U3X

Structure of a human VH antibody domain binding to the cleft of hen egg lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	68.070, 39.240, 100.180
Unit cell angles	90.00, 104.16, 90.00

Refinement procedure

Resolution	97.140 - 2.260
R-factor	0.1923
Rwork	0.189
R-free	0.24839
Starting model (for MR)	1lyz 1ohq
RMSD bond length	0.016
RMSD bond angle	1.709
Refinement software	REFMAC (5.8.0071)

Data quality characteristics

	Overall
Low resolution limit [Å]	97.140
High resolution limit [Å]	2.260
Number of reflections	24456
<I/σ(I)>	2.4
Completeness [%]	98.6
Redundancy	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	300 mM sodium citrate, 16% PEG 3350