4U3S
Crystal structure of Coh3ScaB-XDoc_M1ScaA complex: A N-terminal interface mutant of type II Cohesin-X-Dockerin complex from Acetivibrio cellulolyticus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-20 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97620 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 72.320, 72.320, 231.490 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 62.630 | 62.630 | 1.680 |
High resolution limit [Å] | 1.640 | 7.330 | 1.640 |
Rmerge | 0.113 | 0.043 | 0.702 |
Rmeas | 0.117 | 0.045 | 0.725 |
Total number of observations | 1230010 | ||
Number of reflections | 45113 | 949 | 6166 |
<I/σ(I)> | 13.46 | 26.35 | 3.24 |
Completeness [%] | 100.0 | 99.7 | 100 |
Redundancy | 26.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 292 | 0.1 M CHES pH 9.5, 20% v/v PEG 8000 |