4U3O
Octameric RNA duplex soaked in manganese(II)chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.37 |
| Spacegroup name | H 3 |
| Unit cell lengths | 46.328, 46.328, 58.075 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.160 - 1.800 |
| R-factor | 0.1654 |
| Rwork | 0.164 |
| R-free | 0.17540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u3l |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.913 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.160 | 1.843 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.058 | 0.218 |
| Number of reflections | 4140 | |
| <I/σ(I)> | 43.26 | 12.06 |
| Completeness [%] | 95.7 | 79.91 |
| Redundancy | 19 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 28% PEG 400, 0.2 M calcium chloride, 0.002 M spermine, 0.05 M hepes sodium buffer, pH 7.5 |
