4U3M
Crystal structure of Anisomycin bound to the yeast 80S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 436.680, 287.990, 304.760 |
Unit cell angles | 90.00, 99.01, 90.00 |
Refinement procedure
Resolution | 99.795 - 3.000 |
R-factor | 0.1994 |
Rwork | 0.199 |
R-free | 0.24530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3U5B 3U5C 3U5D 3U5E 3U5F 3U5G 3U5H 3U5I |
RMSD bond length | 0.013 |
RMSD bond angle | 1.700 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX ((phenix.refine: dev_1702)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 99.795 | 3.100 | |
High resolution limit [Å] | 3.000 | 150.000 | 3.000 |
Rmerge | 0.300 | 2.747 | |
Rmeas | 0.311 | 2.860 | |
Total number of observations | 20088881 | ||
Number of reflections | 1479440 | 138204 | |
<I/σ(I)> | 8.36 | 1.09 | |
Completeness [%] | 100.0 | 100 | |
Redundancy | 12.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine |