4U38
RNA duplex containing UU mispair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2012-05-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.028, 81.309, 36.669 |
| Unit cell angles | 90.00, 113.08, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.21345 |
| Rwork | 0.211 |
| R-free | 0.25198 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 434d |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.842 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.166 | 0.355 |
| Number of reflections | 12816 | |
| <I/σ(I)> | 10.2 | 2 |
| Completeness [%] | 93.1 | 76.6 |
| Redundancy | 6.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10% MPD, 40 mM Na cacodylate pH 7.0, 12 mM spermine tetra-HCl, 80 mM KCl and 20 mM BaCl2 |
