4U34
Crystal Structures of RNA Duplexes Containing 2-thio-Uridine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2012-04-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 37.385, 38.683, 30.072 |
Unit cell angles | 90.00, 109.18, 90.00 |
Refinement procedure
Resolution | 22.060 - 1.350 |
R-factor | 0.18807 |
Rwork | 0.188 |
R-free | 0.19788 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 434d |
RMSD bond length | 0.005 |
RMSD bond angle | 1.412 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.400 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.065 | 0.129 |
Number of reflections | 8031 | |
<I/σ(I)> | 24.3 | 11.1 |
Completeness [%] | 93.5 | 66.6 |
Redundancy | 6.7 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 10% MPD, 40 mM Na cacodylate pH 6.0, 12 mM spermine tetra-HCl and 80 mM NaCl |