4U33
Structure of Mtb GlgE bound to maltose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.980 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 343.231, 242.601, 243.672 |
Unit cell angles | 90.00, 135.15, 90.00 |
Refinement procedure
Resolution | 47.638 - 3.293 |
R-factor | 0.1952 |
Rwork | 0.194 |
R-free | 0.22200 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.415 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.200 |
Number of reflections | 210465 |
<I/σ(I)> | 15 |
Completeness [%] | 99.9 |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.5 and 1.0 M ammonium formate |