4U33

Structure of Mtb GlgE bound to maltose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.980
Spacegroup name	C 1 2 1
Unit cell lengths	343.231, 242.601, 243.672
Unit cell angles	90.00, 135.15, 90.00

Refinement procedure

Resolution	47.638 - 3.293
R-factor	0.1952
Rwork	0.194
R-free	0.22200
RMSD bond length	0.016
RMSD bond angle	1.415
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.200
Number of reflections	210465
<I/σ(I)>	15
Completeness [%]	99.9
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M HEPES pH 7.5 and 1.0 M ammonium formate