4U2P

Full-length AMPA subtype ionotropic glutamate receptor GluA2 in the apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	107.810, 149.130, 352.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.002 - 3.239
R-factor	0.2522
Rwork	0.250
R-free	0.28760
RMSD bond length	0.004
RMSD bond angle	0.927
Data reduction software	XDS
Data scaling software	Aimless (3.14)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.002
High resolution limit [Å]	3.200
Number of reflections	65917
<I/σ(I)>	7.77
Completeness [%]	96.6
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1 M MES, 5.8-6.2% PEG6000, 5% TMAO, 0.1-0.3 M sodium acetate