4U2J

N-terminal domain of C. Reinhardtii SAS-6 homolog bld12p variant Q93E F145W (NN27)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2011-09-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	40.150, 97.000, 142.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.241 - 2.000
R-factor	0.1938
Rwork	0.191
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.769
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.241	2.050
High resolution limit [Å]	2.000	2.000
Rmerge		1.116
Number of reflections	38651
<I/σ(I)>	15.66	2.14
Completeness [%]	100.0	100
Redundancy	7.3	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	293	PEG3350, MES-NaOH