4U28

Crystal structure of apo Phosphoribosyl isomerase A from Streptomyces sviceus ATCC 29083

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97927
Spacegroup name	P 65 2 2
Unit cell lengths	69.111, 69.111, 175.036
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.630 - 1.330
R-factor	0.1283
Rwork	0.127
R-free	0.15100
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.247
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.8.0071)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.350
High resolution limit [Å]	1.330	3.610	1.330
Rmerge	0.071	0.044	0.834
Total number of observations	526343
Number of reflections	57655
<I/σ(I)>	10.9		1.82
Completeness [%]	99.0	94.4	97.9
Redundancy	9.1	11.9	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	289	1.8M Na/K phosphate