4U26
Crystal structure of the E. coli ribosome bound to dalfopristin and quinupristin.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 211.260, 432.340, 621.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.080 - 2.800 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.27090 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.872 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1160)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.900 | |
| High resolution limit [Å] | 2.800 | 70.000 | 2.800 |
| Rmerge | 0.136 | 1.191 | |
| Rmeas | 0.159 | 1.432 | |
| Total number of observations | 3948328 | ||
| Number of reflections | 1296827 | 120009 | |
| <I/σ(I)> | 6.62 | 0.56 | |
| Completeness [%] | 94.1 | 87.6 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 291 | PEG8k, MPD |
| 1 | MICROBATCH | 6.5 | 291 | PEG8k, MPD |
