4U25

Crystal structure of the E. coli ribosome bound to virginiamycin M1.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-29
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	210.640, 434.610, 625.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.413 - 2.900
R-factor	0.2232
Rwork	0.223
R-free	0.26470
RMSD bond length	0.006
RMSD bond angle	1.094
Data reduction software	XDS
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.000		3.000
High resolution limit [Å]	2.900	50.000	2.900
Rmerge	0.155	0.046	0.752
Rmeas	0.197	0.053	0.996
Total number of observations	2375603
Number of reflections	1127134	116	97063
<I/σ(I)>	3.89	12.54	0.55
Completeness [%]	90.1	37.2	80.6
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	291	PEG8k, MPD