4U24
Crystal structure of the E. coli ribosome bound to dalfopristin.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 210.210, 433.030, 619.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.968 - 2.900 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.26390 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.069 |
| Data reduction software | XDS |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 70.000 | 3.000 | |
| High resolution limit [Å] | 2.900 | 50.000 | 2.900 |
| Rmerge | 0.124 | 0.769 | |
| Rmeas | 0.144 | 0.935 | |
| Total number of observations | 3534355 | ||
| Number of reflections | 1169643 | 103248 | |
| <I/σ(I)> | 7.04 | 0.63 | |
| Completeness [%] | 94.9 | 87.1 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.5 | 291 | PEG8k, MPD |
| 1 | MICROBATCH | 6.5 | 291 | PEG8k, MPD |
