4U23
GluA2flip sLBD complexed with FW and (R,R)-2b crystal form F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 60.090, 91.290, 111.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.266 - 1.673 |
R-factor | 0.2198 |
Rwork | 0.218 |
R-free | 0.24990 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.270 |
High resolution limit [Å] | 1.670 |
Number of reflections | 35750 |
<I/σ(I)> | 22 |
Completeness [%] | 99.6 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M bicine/Trizma base, pH 8.5, 0.09 M Morpheus nitrate-phosphate-sulfate, 10% w/v PEG8K, 20% (v/v) ethylene glycol |