4U20

Crystal structure of the E. coli ribosome bound to flopristin.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	211.790, 433.060, 623.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.327 - 2.900
R-factor	0.2348
Rwork	0.235
R-free	0.27850
RMSD bond length	0.006
RMSD bond angle	1.113
Data reduction software	XDS
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.000		3.000
High resolution limit [Å]	2.900	50.000	2.900
Rmerge	0.123	0.045	0.600
Rmeas	0.157	0.052	0.767
Total number of observations	2259195
Number of reflections	1094024	104	90498
<I/σ(I)>	4.39	13.45	0.7
Completeness [%]	87.4	33.4	75.3
Redundancy	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	291	PEG8k, MPD
1	MICROBATCH	6.5	291	PEG8k, MPD