4U1V

Crystal structure of the E. coli ribosome bound to linopristin.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-12
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	211.490, 433.900, 621.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.146 - 3.000
R-factor	0.2247
Rwork	0.225
R-free	0.27420
RMSD bond length	0.007
RMSD bond angle	1.209
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.000		3.100
High resolution limit [Å]	3.000	50.000	3.000
Rmerge	0.131	0.055	0.860
Rmeas	0.154	0.061	1.076
Total number of observations	2887066
Number of reflections	1017173	121	90558
<I/σ(I)>	5.13	26.38	0.66
Completeness [%]	90.2	39	86.2
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	291	PEG8k, MPD
1	MICROBATCH	6.5	291	PEG8k, MPD