4U1U

Crystal structure of the E. coli ribosome bound to quinupristin.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-29
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	211.080, 432.730, 631.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.678 - 2.950
R-factor	0.2486
Rwork	0.248
R-free	0.28190
RMSD bond length	0.006
RMSD bond angle	1.159
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.000		3.000
High resolution limit [Å]	2.950	50.000	2.950
Rmerge	0.107	0.027	0.649
Rmeas	0.143	0.033	0.887
Total number of observations	2016698
Number of reflections	1119166	119	51778
<I/σ(I)>	5.04	18.54	0.67
Completeness [%]	93.3	37.9	88.3
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	291	PEG8k, MPD