4U1F

Crystal structure of middle domain of eukaryotic translation initiation factor eIF3b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-01
Detector	PSI PILATUS 6M
Wavelength(s)	1.00, 0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	60.160, 175.870, 71.320
Unit cell angles	90.00, 113.62, 90.00

Refinement procedure

Resolution	43.637 - 2.200
R-factor	0.1895
Rwork	0.187
R-free	0.22620
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.011
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.200	2.200
Rmerge	0.034	0.064
Number of reflections	67154
<I/σ(I)>	18.51	1.83
Completeness [%]	97.6	97.2
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	PEG MME 5000, potassium thiocyanate, CHES