4U1F
Crystal structure of middle domain of eukaryotic translation initiation factor eIF3b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-04-01 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.00, 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.160, 175.870, 71.320 |
Unit cell angles | 90.00, 113.62, 90.00 |
Refinement procedure
Resolution | 43.637 - 2.200 |
R-factor | 0.1895 |
Rwork | 0.187 |
R-free | 0.22620 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.011 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.034 | 0.064 |
Number of reflections | 67154 | |
<I/σ(I)> | 18.51 | 1.83 |
Completeness [%] | 97.6 | 97.2 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | PEG MME 5000, potassium thiocyanate, CHES |