4U1E

Crystal structure of the eIF3b-CTD/eIF3i/eIF3g-NTD translation initiation complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-27
Detector	PSI PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	C 2 2 21
Unit cell lengths	54.270, 130.290, 192.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.490 - 2.000
R-factor	0.1874
Rwork	0.186
R-free	0.21620
RMSD bond length	0.006
RMSD bond angle	0.954
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Inner shell
Low resolution limit [Å]	50.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.088
Number of reflections	46648
<I/σ(I)>	21.25	1.94
Completeness [%]	99.8	99.8
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		292	PEG 20000, PEG MME 550, imidazole, MES