4U1D
Structure of the PCI domain of translation initiation factor eIF3a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2012-09-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 242.530, 158.270, 93.740 |
Unit cell angles | 90.00, 104.21, 90.00 |
Refinement procedure
Resolution | 39.568 - 3.300 |
R-factor | 0.1719 |
Rwork | 0.170 |
R-free | 0.20370 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.911 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.500 |
High resolution limit [Å] | 3.294 | 3.294 |
Number of reflections | 48944 | |
<I/σ(I)> | 11.14 | 1.53 |
Completeness [%] | 94.2 | 92.6 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | PEG 3350, KSCN, Bis-Tris-propane |