4U1D

Structure of the PCI domain of translation initiation factor eIF3a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	90
Detector technology	PIXEL
Collection date	2012-09-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	242.530, 158.270, 93.740
Unit cell angles	90.00, 104.21, 90.00

Refinement procedure

Resolution	39.568 - 3.300
R-factor	0.1719
Rwork	0.170
R-free	0.20370
RMSD bond length	0.005
RMSD bond angle	0.911
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.500
High resolution limit [Å]	3.294	3.294
Number of reflections	48944
<I/σ(I)>	11.14	1.53
Completeness [%]	94.2	92.6
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	PEG 3350, KSCN, Bis-Tris-propane