4U0Y
Crystal structure of the DNA-binding domains of YvoA in complex with palindromic operator DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-08 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 31 |
| Unit cell lengths | 48.554, 48.554, 153.941 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.520 - 1.910 |
| R-factor | 0.1809 |
| Rwork | 0.178 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DNA-binding domain of PDB entry 2wv0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.087 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.520 | 1.980 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.085 | 0.870 |
| Number of reflections | 31230 | |
| <I/σ(I)> | 10.61 | 1.9 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292.15 | 50 mM sodium cacodylate, 200 mM sodium citrate, 10 mM MgCl2, 5 % (v/v) isopropanol |
