4U0C

Hexameric HIV-1 CA in complex with Nup153 peptide, P6 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-20
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.92
Spacegroup name	P 6
Unit cell lengths	91.469, 91.469, 57.001
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.670 - 1.770
R-factor	0.1923
Rwork	0.191
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h47
RMSD bond length	0.005
RMSD bond angle	0.878
Data reduction software	MOSFLM
Data scaling software	Aimless (0.2.18)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.670	35.670	1.810
High resolution limit [Å]	1.770	9.050	1.770
Rmerge	0.109	0.033	0.942
Rpim	0.066	0.019	0.581
Total number of observations	79253	745	4573
Number of reflections	24947
<I/σ(I)>	7.3	21.4	1.6
Completeness [%]	95.1	89.1	95.8
Redundancy	3.2	4	3.2
CC(1/2)	0.996	0.999	0.392

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	290	30% v/v PEG 400, 0.1 M CHES