4U09
Structure of Leptospira interrogans LRR protein LIC12759
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.28199 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.166, 104.796, 116.597 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.790 - 1.950 |
| R-factor | 0.1873 |
| Rwork | 0.187 |
| R-free | 0.21320 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.790 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.097 | 0.974 |
| Number of reflections | 67851 | |
| <I/σ(I)> | 13.6 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.9 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG 4000, 125 mM zinc acetate, 200 mM magnesium chloride, 100 mM MES |
