4U08
Structure of Leptospira interrogans LRR protein LIC11098
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 105.696, 105.696, 161.723 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.450 - 1.950 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u09 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.450 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.096 | 0.962 |
| Number of reflections | 76748 | |
| <I/σ(I)> | 11.8 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.3 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | PEG 8000, 200 mM calcium acetate, 10mM zinc acetate, 100 mM cacodylate |
