4U06
Structure of Leptospira interrogans LRR protein LIC10831
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-20 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.28199 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 122.300, 122.300, 58.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.200 - 1.900 |
| R-factor | 0.2027 |
| Rwork | 0.201 |
| R-free | 0.23550 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.230 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.053 | 0.627 |
| Number of reflections | 35305 | |
| <I/σ(I)> | 16.1 | 2.1 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 6.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG 4000, 2-propanol, 10mM zinc acetate, 100mM HEPES-Na |
