4TZW
Co-crystals of the Ternary Complex Containing a T-box Stem I RNA, its Cognate tRNAGly, and B. subtilis YbxF protein, treated by removing lithium sulfate and replacing Mg2+ with Sr2+ post crystallization
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-31 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 75.316, 75.316, 268.942 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.786 - 4.671 |
| R-factor | 0.2711 |
| Rwork | 0.264 |
| R-free | 0.33120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lck |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.681 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.790 | 4.840 |
| High resolution limit [Å] | 4.670 | 4.670 |
| Rmerge | 0.052 | 0.990 |
| Number of reflections | 4428 | |
| <I/σ(I)> | 15.2 | 1.9 |
| Completeness [%] | 98.2 | 99 |
| Redundancy | 6.7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM Bis-Tris (HCl) pH 6.5, 300 mM Li2SO4, and 20% (w/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
