4TZV
Co-crystals of the Ternary Complex Containing a T-box Stem I RNA, its Cognate tRNAGly, and B. subtilis YbxF protein, treated by removing lithium sulfate post crystallization
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 75.742, 75.742, 270.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.030 - 5.030 |
| R-factor | 0.2822 |
| Rwork | 0.278 |
| R-free | 0.32480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lck |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.272 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.030 | 5.200 |
| High resolution limit [Å] | 5.030 | 5.030 |
| Rmerge | 0.076 | 1.828 |
| Number of reflections | 3756 | |
| <I/σ(I)> | 13.5 | 1.5 |
| Completeness [%] | 99.8 | 98.9 |
| Redundancy | 13 | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM Bis-Tris (HCl) pH 6.5, 300 mM Li2SO4, and 20% (w/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
