4TZQ

Structure of C. elegans HTP-1 bound to HTP-3 motif-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	.979
Spacegroup name	P 1 21 1
Unit cell lengths	53.073, 66.400, 84.130
Unit cell angles	90.00, 105.58, 90.00

Refinement procedure

Resolution	38.792 - 2.300
R-factor	0.243
Rwork	0.241
R-free	0.27930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tzo
RMSD bond length	0.003
RMSD bond angle	0.768
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	24480
<I/σ(I)>	14.2
Completeness [%]	97.3
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298.15	15-22% PEG 3350