4TZO
Structure of C. elegans HTP-1 bound to HIM-3 closure motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | .979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.897, 107.873, 82.968 |
Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
Resolution | 45.221 - 2.400 |
R-factor | 0.2475 |
Rwork | 0.246 |
R-free | 0.28220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tzl |
RMSD bond length | 0.004 |
RMSD bond angle | 0.770 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 43476 |
<I/σ(I)> | 6.3 |
Completeness [%] | 94.1 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | 15-22% PEG 3350 |