4TZN
Structure of HTP-2 bound to HTP-3 motif-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | .979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 165.820, 38.310, 103.820 |
Unit cell angles | 90.00, 126.24, 90.00 |
Refinement procedure
Resolution | 80.000 - 3.115 |
R-factor | 0.282 |
Rwork | 0.280 |
R-free | 0.31980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tzl |
RMSD bond length | 0.003 |
RMSD bond angle | 0.604 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 80.000 |
High resolution limit [Å] | 3.100 |
Number of reflections | 8218 |
<I/σ(I)> | 8.7 |
Completeness [%] | 83.4 |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298.15 | 15-22% PEG 3350 |