4TZJ

Structure of C. elegans HIM-3 bound to HTP-3 closure motif-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	H 3 2
Unit cell lengths	161.791, 161.791, 110.545
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.239 - 2.851
R-factor	0.1802
Rwork	0.178
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4trk
RMSD bond length	0.004
RMSD bond angle	0.839
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.850
Number of reflections	12444
<I/σ(I)>	20.1
Completeness [%]	95.7
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.75	298.15	15-22% PEG 3350