4TZA
TGP, an extremely thermostable green fluorescent protein created by structure-guided surface engineering
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-09-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.542 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 94.200, 141.220, 69.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.586 - 1.900 |
R-factor | 0.174538516473 |
Rwork | 0.174 |
R-free | 0.20031 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.234 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX ((phenix.refine: 1.9_1685)) |
Refinement software | PHENIX ((phenix.refine: 1.9_1685)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.600 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.062 |
Number of reflections | 72746 |
<I/σ(I)> | 12.3 |
Completeness [%] | 99.2 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% w/v PEG MME 2000, 0.1 M Tris-HCl pH 7.0 |