4TZ7
Crystal structure of type I phosphatidylinositol 4-phosphate 5-kinase alpha from Zebrafish
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2012-02-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.075 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 88.946, 88.946, 157.074 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.309 - 3.310 |
R-factor | 0.21261 |
Rwork | 0.207 |
R-free | 0.26766 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.404 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 3.300 |
Number of reflections | 8877 |
<I/σ(I)> | 30.1 |
Completeness [%] | 99.5 |
Redundancy | 13.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PEG 20,000 |