4TZ0
DEAD-box helicase Mss116 bound to ssRNA and GDP-BeF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 89.979, 126.614, 55.553 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.270 - 2.350 |
R-factor | 0.2324 |
Rwork | 0.231 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.710 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.270 |
High resolution limit [Å] | 2.350 |
Rmerge | 0.133 |
Number of reflections | 27147 |
<I/σ(I)> | 7 |
Completeness [%] | 99.9 |
Redundancy | 19.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 4 % tacsimate, pH 8.0, 12 % PEG 3350 |