4TYZ
Crystal structure of the C-terminal domain of an unknown protein from Leishmania infantum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-13 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 58.860, 58.860, 135.120 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.752 - 1.600 |
R-factor | 0.16 |
Rwork | 0.159 |
R-free | 0.18620 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.022 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1738)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.640 |
High resolution limit [Å] | 1.600 | 7.160 | 1.600 |
Rmerge | 0.048 | 0.017 | 0.522 |
Rmeas | 0.052 | 0.019 | 0.562 |
Total number of observations | 265399 | ||
Number of reflections | 36500 | 443 | 2669 |
<I/σ(I)> | 30.88 | 83.99 | 3.73 |
Completeness [%] | 99.4 | 88.4 | 100 |
Redundancy | 7.3 | 7.29 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 290 | Reagents JCSG+ sceen b3: 20% PEG 6000, 100mM Bicine pH 9.0; LeinA.18749.a.B1.PS02018 at 8.28mg/ml |
2 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | RigakuReagents JCSG+ sceen a9: 20% PEG 3350, 200mM Ammonium chloride; LeinA.18749.a.B1.PS02018 at 8.28mg/ml; the crystal was soaked for 10sec each in reservoir solution with 10% EG/500mM NaI, 20% EG/1000mM NaI, this sample was used for in-house phasing |