4TYY

DEAD-box helicase Mss116 bound to ssRNA and CDP-BeF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	89.635, 126.842, 55.303
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.066 - 2.740
R-factor	0.2256
Rwork	0.223
R-free	0.26680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i5x
RMSD bond length	0.003
RMSD bond angle	0.629
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.066	2.800
High resolution limit [Å]	2.740	2.750
Rmerge	0.127	0.595
Number of reflections	16982
<I/σ(I)>	8.9	2.94
Completeness [%]	98.6	100
Redundancy	6.3	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295	0.2 M sodium malonate, pH 5.0, 20 % PEG 3350